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Analysis of polymeric proteins by two‐dimensional polyacrylamide gel electrophoresis on one slab in the presence and absence of sodium dodecyl sulfate
Author(s) -
Bloemendal Hans,
Jansen Kees
Publication year - 1986
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150070808
Subject(s) - sodium dodecyl sulfate , electrophoresis , gel electrophoresis , chromatography , chemistry , polyacrylamide gel electrophoresis , gel electrophoresis of proteins , sodium , slab , two dimensional gel electrophoresis , polyacrylamide , biochemistry , polymer chemistry , biology , enzyme , organic chemistry , proteomics , paleontology , gene
The method presented was developed specifically to be used for the electrophoretic analysis of polymeric proteins in order to establish the number of aggregates and that of their subunits. Electrophoresis is carried out on a single slab; without sodium dodecyl sulfate (SDS) in the first dimension and with SDS in the second dimension. The method is possible since for one‐dimensional SDS gel electrophoresis, SDS is only required in the cathode vessel.