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Decrease in the mobility of proteins during electrophoresis in linear gradients of polyacrylamide
Author(s) -
Lambin Patrick,
Herance Nicole,
Fine JeanMarius
Publication year - 1986
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150070711
Subject(s) - electrophoresis , logarithm , polyacrylamide , polyacrylamide gel electrophoresis , chemistry , chromatography , linear correlation , linear relationship , gel electrophoresis of proteins , gel electrophoresis , analytical chemistry (journal) , biochemistry , mathematics , enzyme , statistics , mathematical analysis , polymer chemistry
Proteins with molecular weight between 66 000 and 950 000 were subjected to electrophoresis in 3 to 30 % linear gradients of polyacrylamide with volt‐hour products between 2000 and 20 000. From the experimental data it was concluded that displacement of proteins decreased proportionally to the logarithm of time of electrophoresis. With these coordinates the fitness of the curves was found to be excellent (correlation coefficients were better than 0.993 for the analyzed proteins). These results support the hypothesis that there is no limit to be reached by a protein during its migration in a linear gradient of polyacrylamide. In addition we observed that the rate of decrease in mobility was proportional to the size of the protein.