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Mathematical simulation of isotachophoresis boundary between protein and weak acid
Author(s) -
Shimao Kazuo
Publication year - 1986
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150070703
Subject(s) - isotachophoresis , stacking , molecule , boundary (topology) , gaussian , chemistry , boundary value problem , chemical physics , chromatography , biological system , computational chemistry , mathematical analysis , mathematics , organic chemistry , biology , electrolyte , electrode
Mathematical simulation of an isotachophoretic boundary between protein and weak acid was made on the assumption of steady state. In order to deal with a protein molecule, a Gaussian model for charge distribution of protein molecule was constructed, based on the assumption that acidic and basic residues on a protein molecule dissociate independently of each other. The results were roughly in agreement with protein stacking experiments with regard to boundary thickness and concentration of the stacked zone.