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A technical note on esterase D subtyping
Author(s) -
Rocha Jorge,
Amorim AntóNio
Publication year - 1986
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150070610
Subject(s) - subtyping , isoelectric focusing , electrophoresis , agarose , chromatography , esterase , chemistry , phosphate buffered saline , buffer (optical fiber) , isoelectric point , computer science , biochemistry , enzyme , telecommunications , programming language
The electrophoretic behavior of esterase D (E.C. 3.1.1.1.) allozymes 1, 2 and 5 was studied in various buffers and pH ranges, and compared with results obtained on isoelectric focusing. It is shown that the relative mobility of ESD 2 and 5 allozymes is reversed according to the pH of the buffer system. In acidic pH ranges the mobility is: cathode 1<2<5 anode; using the same buffer system in basic pH ranges the following order is observed: 1<5<2. Technical improvements in agarose gel electrophoresis leading to a considerable economical advantage over the previously described methods are presented.