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The use of biotinylated DNA probes for detecting single copy human restriction fragment length polymorphisms separated by electrophoresis
Author(s) -
Dykes Dale,
Fondell Joseph,
Watkins Paul,
Polesky Herbert
Publication year - 1986
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150070607
Subject(s) - restriction enzyme , microbiology and biotechnology , streptavidin , biotinylation , dna , genomic dna , restriction fragment length polymorphism , southern blot , nick translation , biology , hybridization probe , biotin , restriction fragment , molecular probe , gel electrophoresis , polymerase chain reaction , genetics , gene
Human genomic DNA probes labeled by nick translation with biotin 11‐UTP were successfully used to identify single copy restriction fragment length polymorphisms following electrophoresis and Southern blotting onto nylon membranes. Two human probes found on chromosome 21 were used, each of which demonstrated polymorphisms using two separate restriction endonucleases. Compared to isotopic labeling of probes the biotin labels are safer, faster and more economical. The detection method of streptavidin‐biotin‐alkaline phosphatase permitted phenotyping in a matter of hours after hybridization of the probe to the human DNA fragments.