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A new applicator strip with a concentration effect for isoelectric focusing
Author(s) -
Pflug Werner,
Laczko Bela,
Müller Fritz
Publication year - 1986
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150070511
Subject(s) - isoelectric focusing , strips , phosphoglucomutase , chromatography , distortion (music) , sensitivity (control systems) , analytical chemistry (journal) , chemistry , materials science , immobilized ph gradient , optoelectronics , composite material , engineering , electronic engineering , amplifier , biochemistry , cmos , enzyme
The sensitivity for protein detection after isoelectric focusing can be enhanced using a newly developed applicator strip with a concentration effect. This effect is achieved with funnel‐shaped slots, which are placed parallel to the pH gradient. Furthermore, sample volumes varying from 1–90 μL can be applied without distortion to the pH gradient. A comparison with other commercially available applicator strips was undertaken using the phosphoglucomutase system.