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Fast silver staining of polyacrylamide gels at elevated temperatures
Author(s) -
Biel Hans J.,
Gronski Peter,
Seiler Fritz R.
Publication year - 1986
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150070510
Subject(s) - polyacrylamide , silver nitrate , silver stain , coomassie brilliant blue , staining , chromatography , chemistry , polyacrylamide gel electrophoresis , acetic acid , nuclear chemistry , biochemistry , polymer chemistry , biology , microbiology and biotechnology , genetics , enzyme
Renouncing protein fixation with aldehydes, the polyacrylamide gels are incubated in alcoholic solutions of silver nitrate containing acetic acid at 60–70 °C. for a short time and developed with NaOH, also at 60–70 °C. Using various methods of protein fixation, the detection limits of human albumin were determined for 0.75 and 1.5 mm thick polyacrylamide gels with appropriate thickness correction. An overlay technique with silver nitrate containing polyacrylamide gels is described. Dispersing Coomassie Brilliant Blue G‐250 in a solution containing HNO 3 and NH 4 NO 3 allows rapid silver staining of prestained gels.