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Mitochondrial DNA in natural populations: An improved routine for the screening of genetic variation based on sensitive silver staining
Author(s) -
Tegelström Håkan
Publication year - 1986
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150070508
Subject(s) - mitochondrial dna , silver stain , dna , dna extraction , staining , biology , restriction fragment length polymorphism , base pair , microbiology and biotechnology , genetics , polymerase chain reaction , gene
When screening natural populations for genetic variation in mitochondrial (mt) DNA it is necessary to screen as many individuals as possible for as large a number of base pairs as possible. This report describes a combination of laboratory techniques – isolation of mtDNA by either density ultracentrifugation or a phenol extraction procedure and DNA fragment visualization by a sensitive silver staining technique. These routines remove the major restriction of tissue sample size and number of individuals that can be screened and allows the investigation of at least 1000 base pairs from 1 g of tissue or 3–400 base pairs from 100 mg of frozen tissue.