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A device for preparative elution electrophoresis using a polyacrylamide gel slab
Author(s) -
Carpenter Helen C.,
Skerritt John H.,
Wrigley Colin W.,
Margolis Joel
Publication year - 1986
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150070507
Subject(s) - elution , chromatography , electrophoresis , polyacrylamide gel electrophoresis , chemistry , gel electrophoresis , polyacrylamide , resolution (logic) , sodium dodecyl sulfate , gel electrophoresis of proteins , free flow electrophoresis , gel electrophoresis of nucleic acids , biochemistry , enzyme , computer science , artificial intelligence , polymer chemistry
A compact device, capable of being clipped onto the end of a 3 mm thick analytical polyacrylamide slab gel, has been developed for the collection of resolved protein zones as they elute from the end of the gel. Its unique feature is that eluted proteins were collected between two polyacrylamide‐embedded paper membranes and removed either by continuous or intermittent buffer elution. Because of this direct coupling to large gels identical to those used for analytical electrophoresis, protein zones were obtained with high resolution. The method was applicable to non‐denaturing cathodic and anodic electrophoresis, as well as sodium dodecyl sulfate electrophoresis. Medium‐scale quantities (up to 10 mg/cm 2 cross‐sectional area) of protein were fractionated in as little as 4 h with high recoveries.