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A micromethod for the preparation of tissue from defined fractions of a Drosophila head and its analysis by sodium dodecyl sulfate‐polyacrylamide gel electrophoresis
Author(s) -
Müller Uli,
Spatz HansChristof
Publication year - 1986
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150070504
Subject(s) - coomassie brilliant blue , sodium dodecyl sulfate , polyacrylamide gel electrophoresis , staining , gel electrophoresis , chromatography , sodium , biochemistry , chemistry , electrophoresis , mutant , microbiology and biotechnology , glycoprotein , biology , gene , genetics , organic chemistry , enzyme
A method is described which allows the preparation of retinae, laminae, opticallobes, protocerebrum and proboscis of single heads of Drosophila . The protein content of these tissues was examined with sodium dodecyl sulfate‐polyacrylamide gel electrophoresis followed by Coomassie Brilliant Blue R‐250 and glycoprotein staining. A catalog of 39 major proteins or protein subunits was obtained. Their relative proportions were determined quantitatively for the different tissues from Drosophila wildtype strains AS and C‐S as well as the mutant sev LY3 .