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Subtyping group‐specific component or esterase D using the same ultrathin‐layer polyacrylamide gel format
Author(s) -
Budowle Bruce
Publication year - 1986
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150070307
Subject(s) - subtyping , isoelectric focusing , esterase , typing , polyacrylamide gel electrophoresis , component (thermodynamics) , polyacrylamide , layer (electronics) , chromatography , chemistry , biology , microbiology and biotechnology , genetics , computer science , biochemistry , enzyme , physics , organic chemistry , programming language , thermodynamics
An ultrathin‐layer polyacrylamide isoelectric focusing gel is described for obtaining highly resolved, nondistorted, reproducible patterns for group‐specific component (Gc) or esterase D (EsD) subtyping. The 1F, 1S, 2 and 1A1 bands of the Gc system were all typeable by isoelectric focusing alone. All common EsD allelic products were easily resolved. Typing Gc or EsD derived from bloodstains was also demonstrated.