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Microelectrophoresis in the differential diagnosis of proteinuric diseases
Author(s) -
Weber Michael H.,
Cheong Kim KyungSun,
Schott KlausJoachim,
Neuholff Volker
Publication year - 1986
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150070306
Subject(s) - microelectrophoresis , electrophoresis , gel electrophoresis of proteins , gel electrophoresis , chromatography , polyacrylamide gel electrophoresis , chemistry , molecular weight size marker , difference gel electrophoresis , proteinuria , sodium dodecyl sulfate , microbiology and biotechnology , biochemistry , medicine , biology , proteomics , kidney , enzyme , gene
Micro‐polyacrylamide gel electrophoresis (micro‐PAGE) has been shown to be useful for molecular size separation of urinary proteins. In addition to the sodium dodecyl sulfate micro‐gradient gel electrophoresis in 10 μL capillaries, which is used routinely for examination of proteinuria in our laboratory, we tried micro‐gradient slab gel electrophoresis (micro‐slab‐PAGE) for molecular weight determination of urinary proteins. It was also employed for monitoring patients with proteinuric diseases. For detailed studied of urinary proteins, micro‐two‐dimensional‐electrophoresis is recommended.