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Sample band spreading phenomena in ground and spacebased electrophoretic separators
Author(s) -
Rhodes Perey H.,
Snyder Robert S.
Publication year - 1986
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150070303
Subject(s) - transverse plane , perpendicular , sample (material) , conductivity , electrical resistivity and conductivity , materials science , flow (mathematics) , ground plane , plane (geometry) , optics , analytical chemistry (journal) , mechanics , chemistry , physics , chromatography , geometry , electrical engineering , mathematics , structural engineering , quantum mechanics , antenna (radio) , engineering
Sample stream spreading in the transverse direction (direction perpendicular to the chamber center plane) is calculated for two ground‐based and one space‐based continuous flow electrophoresis chambers. The magnitude of transverse sample stream spreading is shown to be a function of the difference in electrical conductivity between the sample and curtain buffer. Sample/buffer conductivity mismatch distorts the normally uniform electrical field in the vicinity of the sample to produce transverse field components which, in turn, deflect sample material toward the chamber walls. These results indicate that non‐gravity effects are important and must be resolved in order for space‐based continuous flow electrophoresis to achieve its maximum potential.