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Computer analysis of two‐dimensional gels by a general image processing system
Author(s) -
Tyson John J.,
Haralick Robert H.
Publication year - 1986
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150070302
Subject(s) - physarum polycephalum , division (mathematics) , image processing , gaussian , computer science , homogeneous , image (mathematics) , biological system , intensity (physics) , artificial intelligence , pattern recognition (psychology) , chemistry , mathematics , optics , statistical physics , biology , physics , biochemistry , computational chemistry , arithmetic
In order to survey changes in accumulation of several hundred proteins during the naturally synchronous nuclear division cycle of Physarum polycephalum , we have developed methods for analyzing two‐dimensional (2‐D) gel electrophoretograms using the general image processing system developed by the Spatial Data Analysis Laboratory at Virginia Tech. In this paper we describe fast and accurate methods for removing non‐homogeneous background intensity from a 2‐D gel image, for resolving overlapping protein spots, and for estimating the total integrated intensity in a protein spot by Gaussian modeling.