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Ultrathin‐layer isoelectric focusing of the photoaffinity labeled benzodiazepine receptor in calf brain: A new and simple method for the study of integral membrane proteins
Author(s) -
Lentes Klaus Ulrich,
Friedl Waltraut,
Hebebrand Johannes,
Propping Peter
Publication year - 1986
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150070211
Subject(s) - isoelectric focusing , urea , chemistry , membrane , integral membrane protein , chromatography , polyacrylamide , membrane protein , isoelectric point , receptor , polyacrylamide gel electrophoresis , biophysics , biochemistry , biology , polymer chemistry , enzyme
Abstract A new method for analytical isoelectric focusing of the benzodiazepine receptor in calf brain is described. Brain membranes are photoaffinity labeled with a specific radioligand, solubilized in 9 M urea and separated on 150 μm ultrathin polyacrylamide gels containing 8 M urea. The method combines high resolution with speed and versatility. It may be generally applicable for isoelectric focusing of integral membrane proteins in genetic studies.