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On the reproducibility of band position in electrophoretic separations
Author(s) -
Gianazza Elisabetta,
AstruaTestori Silvia,
Caccia Paolo,
Giacon Patrizia,
Quaglia Laura,
Righetti Pier Giorgio
Publication year - 1986
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150070205
Subject(s) - electrophoresis , isoelectric focusing , reproducibility , chromatography , chemistry , sodium dodecyl sulfate , polyacrylamide , analytical chemistry (journal) , sodium , gel electrophoresis , polyacrylamide gel electrophoresis , biochemistry , organic chemistry , polymer chemistry , enzyme
The reproducibility of protein migration was studied in three electrophoretic systems: sodium dodecyl‐sulfate electrophoresis on polyacrylamide gradient, isoelectric focusing on immobilized pH gradients and two‐dimensional charge‐size separations. The gradient gels were poured either with a two‐vessel apparatus or with a set of computer‐driven burettes, as described by Altland and Altland ( Electrophoresis 1984, 5 , 143–147). Standard deviation on band position was cut by 20–50% with the latter approach in comparison with the former. Typical results were: sodium dodecyl sulfate‐polyacrylamide gel electrophoresis, 0.6 mm (over a total length of 135 mm); immobilized pH gradients, 0.6 mm (for 179 mm length); two‐dimensional separations, 0.8 mm (χ‐axis), 0.6 mm ( y ‐axis).