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A modification for “smileless” polyacrylamide gels in nucleic acid sequencing
Author(s) -
Brown Donald J.,
Bos Timothy J.
Publication year - 1986
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150070109
Subject(s) - nucleic acid , polyacrylamide , electrophoresis , polyacrylamide gel electrophoresis , chemistry , interpretation (philosophy) , nucleic acid quantitation , simple (philosophy) , peptide nucleic acid , chromatography , biochemistry , computer science , polymer chemistry , enzyme , philosophy , epistemology , programming language
The interpretation of nucleic acid sequences on polyacrylamide gels is often made difficult due to “smiles”. We have devised a simple modification for commonly used electrophoresis systems to eliminate this problem.