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Identification of rat liver carboxylesterase isozymes (EC 3.1.1.1) using polyacrylamide gel electrophoresis and isoelectric focusing
Author(s) -
Simon Babette,
de Looze Stephen,
Ronai Adam,
von Deimling Otto
Publication year - 1985
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150061202
Subject(s) - carboxylesterase , isoelectric focusing , isozyme , polyacrylamide gel electrophoresis , biology , electrophoresis , biochemistry , polyacrylamide , isoelectric point , gel electrophoresis , microbiology and biotechnology , esterase , chromatography , chemistry , enzyme
The banding pattern of rat liver carboxylesterases (EC 3.1.1.1) was demonstrated following polyacrylamide gel electrophoresis and isoelectric focusing using standardized conditions. Phenotypic variations, occurring in commonly used inbred rat strains, were compared. Separate isozymes were identified using genetic nomenclature. Individual bands were labelled; their electrophoretic parameters were estimated. Three hitherto genetically undefined zones were described and preliminarily classified as carboxylesterase isozymes. A scheme was provided to enable identification of liver esterases in rat strains not investigated in the present study.