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An immunochemical method for detecting an inactive enzyme (bisphosphoglyceromutase) after Cellogel electrophoresis
Author(s) -
Calvin MarieClaude,
Préhu MarieOdette,
Kechemir Dalila,
Villeval JeanLuc,
Rosa Raymonde
Publication year - 1985
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150061111
Subject(s) - electrophoresis , enzyme , antibody , microbiology and biotechnology , chemistry , gel electrophoresis , staining , chromatography , peroxidase , western blot , biochemistry , antigen , biology , immunology , genetics , gene
A new method is described for detecting inactive enzymes or other proteins after Cellogel electrophoresis. The proteins were blotted from the Cellogel strip to a nitro‐cellulose membrane and then the blot was blocked before incubation in a specific antibody solution. The antigen‐antibody complex was located by a second antibody staining procedure employing peroxidase‐linked goat anti‐rabbit IgG. The method described is sensitive and could be of general use for the detection of proteins after Cellogel electrophoresis.