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Sodium dodecyl sulfate agarose gel electrophoresis: Stacking of the samples in a short polyacrylamide layer
Author(s) -
Rhalem Abdelkébir,
Pery Pierre
Publication year - 1985
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150061110
Subject(s) - agarose , sodium dodecyl sulfate , chromatography , chemistry , polyacrylamide , gel electrophoresis , polyacrylamide gel electrophoresis , molecular weight size marker , gel electrophoresis of proteins , stacking , gel electrophoresis of nucleic acids , elution , electrophoresis , color marker , agarose gel electrophoresis , two dimensional gel electrophoresis , biochemistry , enzyme , dna , organic chemistry , polymer chemistry , proteomics , gene
A metod is described for obtaining a sample‐stacking effect before sodium dodecyl sulfate‐agarose gel electrophoresis. The method requires no novel equipment and uses a discontinuous polyacrylamide‐agarose horizontal slab gel system. With the buffers selected, a complex mixture of proteins can be separated, according to the molecular weight of their subunits, in 15 % w/w agarose gels. The new gel system is versatile and may offer advantages over sodium dodecyl sulfate ‐ polyacrylamide gel electrophoresis in such applications as protein elution or enzyme renaturation.