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Thin‐layer counter current line absorption immuno‐electrophoretic analysis of antigens and antibodies to aleutian disease virus ‐ a mink parvovirus
Author(s) -
Alexandersen Søren,
Hau Jann,
Aasted Bent,
Poulsen Otto M.
Publication year - 1985
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150061104
Subject(s) - antigen , electrophoresis , antibody , parvovirus , mink , immunoelectrophoresis , microbiology and biotechnology , virology , virus , chemistry , biology , chromatography , immunology , ecology
A thin‐layer counter current line absorption immunoelectrophoresis (TL‐CCLAIE) assay was developed for the analysis of antigens and antibodies to Aleutian disease virus. The TL‐CCLAIE assay is a rapid and sensitive assay employing 0.4 mm gels cast in moulds in order to allow technicians not routinely engaged in electrophoretic work to perform the assay. Thin gels allow a higher voltage and thus higher electrophoretic mobility of the antigens, making it possible to perform the complete assay within 2 h.