Thin‐layer counter current line absorption immuno‐electrophoretic analysis of antigens and antibodies to aleutian disease virus ‐ a mink parvovirus | Zendy

Alexandersen Søren | Zendy; Hau Jann | Zendy; Aasted Bent | Zendy; Poulsen Otto M. | Zendy

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Thin‐layer counter current line absorption immuno‐electrophoretic analysis of antigens and antibodies to aleutian disease virus ‐ a mink parvovirus

Author(s) -

Alexandersen Søren,

Hau Jann,

Aasted Bent,

Poulsen Otto M.

Publication year - 1985

Publication title -

electrophoresis

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.666

H-Index - 158

eISSN - 1522-2683

pISSN - 0173-0835

DOI - 10.1002/elps.1150061104

Subject(s) - antigen , electrophoresis , antibody , parvovirus , mink , immunoelectrophoresis , microbiology and biotechnology , virology , virus , chemistry , biology , chromatography , immunology , ecology

A thin‐layer counter current line absorption immunoelectrophoresis (TL‐CCLAIE) assay was developed for the analysis of antigens and antibodies to Aleutian disease virus. The TL‐CCLAIE assay is a rapid and sensitive assay employing 0.4 mm gels cast in moulds in order to allow technicians not routinely engaged in electrophoretic work to perform the assay. Thin gels allow a higher voltage and thus higher electrophoretic mobility of the antigens, making it possible to perform the complete assay within 2 h.

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