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FXIIIA Phenotyping by isoelectric focusing and immunoblotting: Gene frequencies in a population of US Whites and Blacks
Author(s) -
Dykes Dale D.,
Polesky Herbert F.
Publication year - 1985
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150061011
Subject(s) - isoelectric focusing , agarose gel electrophoresis , gene , human plasma , allele , population , agarose , microbiology and biotechnology , allele frequency , genetics , biology , chemistry , biochemistry , chromatography , medicine , enzyme , environmental health
Plasma specimens were phenotyped for FXIIIA in local Whites and Blacks using agarose isoelectric focusing in the range pH 4–7, followed by immunoblotting. Gene frequencies for the alleles FXIIIA*1, FXIIIA*2 and FXIIIA*4 in Whites were 0.774, 0.225 and 0.001, respectively. In Blacks FXIIIA*1 was observed at a frequency of 0.771 and FXIIIA*2 at 0.229. The rare phenotype FXIIIA 3‐1, which by conventional electrophoresis demonstrates two bands, was found to clearly show three bands using isoelectric focusing and immunoblotting. The technique used in this study is inexpensive, easy to perform and can differentiate the gene products of all the currently known variants of FXIIIA.