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Background estimation in one‐dimensional electropherograms of whole‐cell protein extracts
Author(s) -
Lasters Ignace,
Leyns Frederik,
Jackman Peter J. H.
Publication year - 1985
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150061008
Subject(s) - electropherogram , smoothing , trace (psycholinguistics) , biological system , chromatography , pattern recognition (psychology) , mathematics , artificial intelligence , chemistry , computer science , statistics , biology , electrophoresis , linguistics , philosophy
In electropherograms of whole‐cell protein extracts a background absorbance due to many partially overlapping bands is usually observed. Since this background is in general not relevant for the identification of a given pattern, its removal will increase the discrimination between patterns, especially when the distance between patterns is measured on a correlation basis. Here we present an algorithm for the estimation of background envelope. The algorithm selects a set of concave kernels of the total profile and subsequently performs a Fourier smoothing of the trace linking these kernels. A fraction of this smoothed trace is then the background estimate. The method is illustrated for the analysis of total protein extracts of bacterial strains.