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Concentration of cerebrospinal fluid does not affect immunoglobulin G oligoclonal banding patterns
Author(s) -
Shapshak Paul,
Tourtellotte Wallace W.,
Lee Mike M.,
Staugaitis Susan M.,
Cowan Tina,
Ingram Tim
Publication year - 1985
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150061007
Subject(s) - isoelectric focusing , cerebrospinal fluid , chromatography , chemistry , collodion , radial immunodiffusion , immunofixation , membrane , immunoglobulin g , antibody , immunology , pathology , biochemistry , medicine , enzyme , monoclonal antibody , monoclonal
Abstract The effects of concentration on immunoglobulin G (IgG) oligoclonal bands after isoelectric focusing in cerebrospinal fluids (CSF) were examined using six concentration procedures, and the percent recoveries of IgG varied. The procedures, % IgG recoveries, and corresponding degrees of concentration are as follows: (1) Amicon CF25 membrane cone, 75 %, 45 ×; (2) Amicon CF50A membrane cone, 65 %, 3 ×; (3) Amicon CS15 Minicon, 47 %, 80 ×; (4) Microprodicon, 55 %, 35 ×; (5) collodion bag negative pressure dialysis, 50 %, 16 ×; and (6) microcollodion bag in Permasorb, 40–80 %, 10–60 ×. Ultracentrifugation of CSF at 100 000 g for 1 h prior to concentration using CF 25 membrane cones introduced not more than 5 % variation in the IgG concentrations. Isoelectric focusing was performed on 20 CSF samples concentrated by CF25 membrane cone, CS15 Minicon, and collodion bag, and no effects on the oligoclonal IgG banding patterns were found.