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Identification map of human plasma proteins: Micro two‐dimensional electrophoresis followed by multiple immunoreplica technique
Author(s) -
Manabe Takashi,
Takahashi Yuko,
Higuchi Noriko,
Okuyama Tsuneo
Publication year - 1985
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150060908
Subject(s) - electroblotting , nitrocellulose , human plasma , chromatography , blot , polyacrylamide gel electrophoresis , electrophoresis , polyacrylamide , antiserum , chemistry , gel electrophoresis of proteins , gel electrophoresis , blood proteins , two dimensional gel electrophoresis , microbiology and biotechnology , biochemistry , biology , proteomics , antibody , genetics , membrane , gene , enzyme
Human plasma proteins were subjected to parallel micro two‐dimensional polyacrylamide gel electrophoresis in the absence of denaturing agents. Forty nitrocellulose blots were obtained from eight slab gels within one hour of electroblotting time, employing an apparatus designed to obtain multiple replicas from micro slab gels. The locations of 31 human plasma proteins were determined on these blots using specific antisera, and an “identification map” was prepared. Differences of protein location between plasma and serum were also demonstrated.