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Protein staining of 6 mm diameter sodium dodecyl sulfate‐polyacrylamide gels within one and a half hours
Author(s) -
An der Lan Birgit C.,
Auzan Colette,
Sullivan James V.,
Chrambach Andreas
Publication year - 1985
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150060812
Subject(s) - sodium dodecyl sulfate , polyacrylamide , coomassie brilliant blue , staining , chromatography , polyacrylamide gel electrophoresis , trichloroacetic acid , chemistry , stain , sodium , gel electrophoresis , biochemistry , biology , polymer chemistry , organic chemistry , genetics , enzyme
A device for rapid rotation of perforated gel holding tubes has been constructed by which the diffusion of sodium dodecyl sulfate (SDS) from cylindrical polyacrylamide gels can be substantially accelerated. This device allows one to stain protein bands obtained in SDS‐polyacrylamide gel electrophoresis (9 % gel concentration) within one and a half hours, using the procedure of fixation in trichloroacetic acid followed by Coomassie Brilliant Blue G‐250 staining, which requires no destaining.