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A comparison of two‐dimensional gel electrophoresis methods for analysis of rat serum proteins following dimethylformamide exposure
Author(s) -
Marshall Thomas,
Williams Katherine M.,
Vesterberg Olof
Publication year - 1985
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150060808
Subject(s) - isoelectric focusing , resolution (logic) , dimethylformamide , electrophoresis , chromatography , chemistry , two dimensional gel electrophoresis , gel electrophoresis , gel electrophoresis of proteins , polyacrylamide gel electrophoresis , biochemistry , proteomics , enzyme , gene , computer science , artificial intelligence , solvent
Abstract Four two‐dimensional electrophoresis methods, each incorporating different protein denaturing/dissociating conditions, have been used to detect changes in rat serum proteins following exposure to dimethylformamide. The methods were evaluated on the basis of (i) their ability to detect changes, (ii) the resolution achieved and (iii) the response of resolution to the use of narrow Ampholine pH ranges in the first dimensional isoelectric focusing gels. All methods reveal minor but reproducible protein changes in response to dimethylformamide. However, high resolution two‐dimensional electrophoresis of the completely dissociated polypeptides reveals most changes and the resolution of this method is most improved by the use of Ampholines of narrow pH range. In general, the number of serum components detected and the resolution achieved is related to the severity of the protein denaturing/dissociating conditions employed. The significance of this observation in relation to others factors likely to influence the choice of two‐dimensional method is discussed.