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Multiple crossed immunoelectrophoresis on agarosecoated GelBond film
Author(s) -
Rochu Daniel,
Fine JeanMarius
Publication year - 1985
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150060807
Subject(s) - agarose , isoelectric focusing , chromatography , immunoelectrophoresis , electrophoresis , cellulose acetate , chemistry , polyacrylamide , membrane , biochemistry , antigen , polymer chemistry , biology , genetics , enzyme
A new technique, multiple crossed immunoelectrophoresis on agarose‐coated GelBond film, is described. A sheet of GelBond with agarose areas containing a single or different antisera can be used for the simultaneous analysis of eight samples. Antigens were separated in the first dimension (i) in an inert medium according to net charge (cellulose acetate membrane elecrophoresis), (ii) in a sieving medium according to charge and molecular size (ultrathin‐layer polyacrylamide gradient gel electrophoresis), or (iii) in a pH gradient according to differences in isoelectric points (ultrathinlayer isoelectric focusing). By using these methods in the first dimension, resolution is improved over that in conventional agarose electrophoresis. The first dimensional strips are easily transferred to the second dimensional antibody‐containing agarose. Use of the GelBond film allows easy handling and convenient storage. Multiple crossed immunoelectrophoresis is a versatile method for rapid analysis of antigenic variability.