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The quantitation of proteins in silver stained polyacrylamide gels
Author(s) -
Yüksel K. Ümit,
Gracy Robert W.
Publication year - 1985
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150060802
Subject(s) - silver stain , isoelectric focusing , staining , polyacrylamide , isoelectric point , chemistry , polyacrylamide gel electrophoresis , differential staining , chromatography , protein subunit , silver nitrate , biochemistry , microbiology and biotechnology , biology , nuclear chemistry , polymer chemistry , genetics , gene , enzyme
This study investigates some of the problems encountered in quantitation of proteins in silver stained polyacrylamide gels. Subunit molecular weight, isoelectric point and amino acid content are examined as possible factors for differential staining intensities of proteins. We also describe a procedure for quantitation using atomic absorption spectroscopy, independent of the colors formed upon silver staining. Using this method we show that some proteins such as pepsin bind silver but fail to develop visual color reactions.