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Sensitive Nitro Blue Tetrazolium staining of proteins in high resolution two‐dimensional gel electrophoresis
Author(s) -
Bahrman Nasser,
Thiellement Hervé
Publication year - 1985
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150060711
Subject(s) - staining , silver stain , coomassie brilliant blue , nitro , polyacrylamide gel electrophoresis , two dimensional gel electrophoresis , gel electrophoresis , electrophoresis , chemistry , polyacrylamide , resolution (logic) , microbiology and biotechnology , gel electrophoresis of proteins , spots , chromatography , biology , biochemistry , enzyme , proteomics , alkyl , genetics , organic chemistry , artificial intelligence , gene , computer science
A new method is described for staining proteins after high resolution two‐dimensional gel electrophoresis. The polyacrylamide gel matrix is stained blue, by photoreduction of Nitro Blue Tetrazolium, while the spots of polypeptides remain unstained. The method is much more sensitive than Coomassie Blue staining and its sensitivity is close to that obtained by silver staining.