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Detection of non‐heme iron proteins following polyacrylamide gel electrophoresis
Author(s) -
Ohmori Daijiro,
Tanaka Yukiko,
Yamakura Fumiyuki,
Suzuki Koji
Publication year - 1985
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150060709
Subject(s) - polyacrylamide gel electrophoresis , heme , chemistry , gel electrophoresis , color marker , gel electrophoresis of proteins , ferredoxin , two dimensional gel electrophoresis , hemeprotein , electrophoresis , polyacrylamide , chromatography , biochemistry , staining , biology , enzyme , proteomics , polymer chemistry , gene , genetics
Non‐heme iron proteins were stainned in the gel with bathophenanthroline disulfonate after polyacrylamide gel electrophoresis. This staining method proved more resistant to color fading than iron detection by α,α′‐dipyridyl, with >50% of the color being retained after 24 h by three ferredoxins of different origin. A linear relationship was found for the amount of iron in non‐heme iron proteins, applied to the gel, and the areas of densitometric tracings.