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Fabric reinforced polyacrylamide gels for electroblotting
Author(s) -
Nishizawa Hideyuki,
Murakami Ayako,
Hayashi Naoko,
Iida Mami,
Abe Yoshihiro
Publication year - 1985
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150060708
Subject(s) - electroblotting , polyacrylamide , nitrocellulose , isoelectric focusing , membrane , isoelectric point , polyacrylamide gel electrophoresis , chromatography , chemistry , polymer , polymer chemistry , biochemistry , organic chemistry , enzyme
Abstract The preparation of 0.5 mm fabric reinforced polyacrylamide (FRP) gels is described. After isoelectric focusing, egg white and isoelectric point marker proteins were quantitatively transferred in only 20 min from the FRP gels to nitrocellulose membranes by electroblotting. FRP gels combine the advantages of previously described supports for polyacrylamide gels with penetrability for electric current during blotting. The gels may be conveniently stored and afford operational versatility.