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A simple method for drying of sodium dodecyl sulfate‐polyacrylamide disc gels
Author(s) -
Aono Sachiko,
Sato Hiroshi,
Semba Reiji,
Kashiwamata Shigeo
Publication year - 1985
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150060611
Subject(s) - polyacrylamide , sodium dodecyl sulfate , chromatography , shrinkage , sodium , polyacrylamide gel electrophoresis , chemistry , filter paper , materials science , polymer chemistry , composite material , biochemistry , organic chemistry , enzyme
A simple method for the drying, on filter paper, of sodium dodecyl sulfate polyacrylamide disc gels is described. This method has been successfully applied to disc gels with polyacrylamide concentrations of 5, 10, and 15%, and also with a linear gradient of 5–15%. The gels dehydrated without any longitudinal shrinkage are suitable for longtime storage and may be used for molecular weight estimation of proteins.