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A simplified procedure for evaluation and storage of isoelectric focusing gels prior to second‐dimension electrophoresis
Author(s) -
Gower David J.,
Tytell Michael
Publication year - 1985
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150060610
Subject(s) - isoelectric focusing , chromatography , polyacrylamide , polyacrylamide gel electrophoresis , stain , isoelectric point , fixation (population genetics) , tube (container) , chemistry , dimension (graph theory) , electrophoresis , materials science , mathematics , staining , biology , biochemistry , composite material , polymer chemistry , gene , genetics , enzyme , pure mathematics
A simple method of stain fixation of polyacrylamide isoelectric focusing tube gels is described. Gel handling and storage are simplified by fixation and second‐dimension quality is markedly improved over that from the original procedure involving quick freezing and frozen storage of the gel.