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Automated, rapid image analysis and densitometry for enzyme linked immunosorbent assay, dot‐blot, and two‐dimensional gels using a microcomputer and electrooptical device
Author(s) -
Lockshin Richard A.
Publication year - 1985
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150060605
Subject(s) - microcomputer , densitometry , dot blot , resolution (logic) , chemistry , enzyme , optical density , chromatography , electrophoresis , analytical chemistry (journal) , materials science , optics , physics , computer science , biochemistry , engineering , artificial intelligence , dna , electrical engineering , chip
A commercially available electrooptical device, coupled to a microcomputer, is capable of analyzing densitometric values with a resolution of 280 × 128 points (35 840 individual points). A complex autoradiogram of a two‐dimensional electrophoretic gel is completely sorted into X and Y coordinates and exposure values differeing by as little as 0.01 O.D. (optical density) units or over an O.D. range of 0 to 4.00 in less than 2 min. For other purposes such as comparison of two gels, immunoelectrophoretic patterns, enzyme linked immunosorbent assay or microtitration plates, dot‐blot assays, or tissue samples, a comparison may be made and all differences stored in the computer in less than one second and further decisions or computer‐driven actions decided on that basis.