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Two‐Dimensional gel electrophoresis of dexamethasoneinduced organ‐specific developmental proteins (fsa,fsb) in rat lung fibroblasts
Author(s) -
Floros Joanna,
Phelps David S.,
Smith Barry T.
Publication year - 1985
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150060508
Subject(s) - lung , fetus , glucocorticoid , secretion , dexamethasone , hormone , biology , microbiology and biotechnology , gel electrophoresis , cell type , cell , medicine , endocrinology , biochemistry , pregnancy , genetics
Glucocorticoids are known to enhance fetal lung maturation. Previous studies with whole lung have shown that specific proteins are enhanced by these hormones. At least two effects of glucocorticoids on fetal lung epithelia require the presence of mesenchymal elements. Since the lung consists of several different cell types, further understanding of glucocorticoid effects on fetal lung protein synthesis requires that such studies be carried out with specific cell types. Using two‐dimensional gel electrophoresis, we have examined the synthesis and secretion of proteins by fetal rat lung fibroblasts in the presence and absence of glucocorticoid. Two proteins, fsa and fsb, are enhanced by dexamethasone, appear to be organ specific, and are enhanced only during prenatal and early postnatal life. They are not enhanced by a variety of other hormones.