Calmodulin binding to nitrocellulose and Zetapore membranes during electrophoretic transfer from polyacrylamide gels | Zendy

RochetteEgly Cécile | Zendy; Daviaud Danièle | Zendy

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Calmodulin binding to nitrocellulose and Zetapore membranes during electrophoretic transfer from polyacrylamide gels

Author(s) -

RochetteEgly Cécile,

Daviaud Danièle

Publication year - 1985

Publication title -

electrophoresis

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.666

H-Index - 158

eISSN - 1522-2683

pISSN - 0173-0835

DOI - 10.1002/elps.1150060507

Subject(s) - nitrocellulose , membrane , polyacrylamide , calmodulin , sodium dodecyl sulfate , chemistry , chromatography , electrophoresis , polyacrylamide gel electrophoresis , sodium , biochemistry , polymer chemistry , organic chemistry , enzyme

In the classical electrotransfer conditions from sodium dodecyl sulfate‐polyacrylamide gels, calmodulin fails to bind to nitrocellulose membranes. However, under conditions favorizing hydrophobic interactions such as a higher salt concentration, calmodulin can be immobilized on nitrocellulose sheets, thus allowing detection in a complex protein mixture.

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