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Calmodulin binding to nitrocellulose and Zetapore membranes during electrophoretic transfer from polyacrylamide gels
Author(s) -
RochetteEgly Cécile,
Daviaud Danièle
Publication year - 1985
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150060507
Subject(s) - nitrocellulose , membrane , polyacrylamide , calmodulin , sodium dodecyl sulfate , chemistry , chromatography , electrophoresis , polyacrylamide gel electrophoresis , sodium , biochemistry , polymer chemistry , organic chemistry , enzyme
In the classical electrotransfer conditions from sodium dodecyl sulfate‐polyacrylamide gels, calmodulin fails to bind to nitrocellulose membranes. However, under conditions favorizing hydrophobic interactions such as a higher salt concentration, calmodulin can be immobilized on nitrocellulose sheets, thus allowing detection in a complex protein mixture.