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Rapid mechanical versus conventional acid fixation in electrophoresis
Author(s) -
Gersten Douglas M.,
Zapolski Edward J.,
Ledley Robert S.
Publication year - 1985
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150060411
Subject(s) - fixation (population genetics) , polyacrylamide gel electrophoresis , polyacrylamide , slab , gel electrophoresis of proteins , chromatography , gel electrophoresis , electrophoresis , chemistry , biochemistry , biology , polymer chemistry , enzyme , gene , paleontology
We have devised methodology which allows for the rapid mechanical fixation of proteins in polyacrylamide slab gels. Proteins so fixed remain in the gel even after four days of soaking. The potential utility of this approach is discussed.