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Experimental evidence for flexible slope of immobilized pH gradients poured under computer control
Author(s) -
Altland Klaus,
Hackler Rolf,
Banzhoff Angelika,
von Eckardstein Arnold
Publication year - 1985
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150060310
Subject(s) - isoelectric focusing , density gradient , immobilized ph gradient , chromatography , flexibility (engineering) , electrophoresis , chemistry , isoelectric point , polyacrylamide , computer science , analytical chemistry (journal) , mathematics , physics , biochemistry , statistics , quantum mechanics , polymer chemistry , enzyme
Recently, Altland and Altland ( Clin. Chem. 1984, 30 , 2098–2103) described three alternative devices for pouring immobilized pH gradients (IPGs) based on simultaneous delivery of a density gradient and a non‐density gradient by using high precision burettes under computer control. One of the expected improvements was an unlimited flexibility for the slope of the non‐density gradient ( e. g. the pH gradient) which is stabilized by the density gradient. The human plasma proteins prealbumin and apolipoprotein AI and some of their inherited variants are demonstrated by double one‐dimensional electrophoresis in the sequence polyacrylamide gel electrophoresis followed by isoelectric focusing (IEF) in IPGs of varying slopes in the range of pH 4 to 7. It is shown that the patterns obtained follow the expectations from the gradients drawn on the computer. We conclude that the new devices for pouring IPGs can provide a valuable tool for the easy handling of complex separation problems to be solved by IEF.