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Simple drying of polyacrylamide gels for fluorography and storage
Author(s) -
Westermann Reiner
Publication year - 1985
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150060308
Subject(s) - polyacrylamide , acetone , chemistry , chromatography , sodium dodecyl sulfate , dehydration , shrinkage , materials science , polymer chemistry , biochemistry , composite material
A simple method for drying polyacrylamide gels of various size (0.3 mm capillary gels to 3 mm thick slab gels) and composition (T = 5–20 %, C = 0.7–5.3 %, sodium dodecyl sulfate gels, acetic acid/urea gels, isoelectric focusing gels) is described. The gels are destained in the presence of 20 % glycerol, dehydrated in acetone, and dried at room temperature. By this procedure, size reduced, clear or slightly opaque, and flexible sheets are obtained. The dried gels can be used for fluorography, stored for long periods and rehydrated without any cracking, warping, or loss in resolution. The shrinkage of the gels to about 25 % of their original size during acetone‐dehydration results in an increased staining intensity and shorter exposure times for fluorography.