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Transferrin subtypes determined by ultrathin‐layer polyacrylamide gel isoelectric focusing
Author(s) -
Budowle Bruce
Publication year - 1985
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150060210
Subject(s) - isoelectric focusing , transferrin , subtyping , polyacrylamide gel electrophoresis , population , chemistry , chromatography , biochemistry , medicine , computer science , enzyme , environmental health , programming language
An ultrathin‐layer polyacrylamide gel isoelectric focusing method is described for subtyping transferrin. After a one‐hour pretreatment at room temperature of transferrin in serum with ferrous ammonium sulfate, the samples were subjected to isoelectric focusing for 140 min with a final voltage of 3500 V. Thus, transferrin subtyping was achieved in one‐half of a working day. Population data on transferrin in Black and White samples from Baltimore, Maryland, were obtained using this method. The data were similar to that previously reported on these races from other geographical locations.