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Electrophoresis of dimethylcyanamidated mercaptoacids
Author(s) -
Wroński Mieczyslaw
Publication year - 1985
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150060208
Subject(s) - electrophoresis , molecule , chemistry , chromatography , cellulose , group (periodic table) , analytical chemistry (journal) , organic chemistry
The conversion of a sulfydryl group of mercaptoacids to a thiuronium group by means of dimethylcynamide followed by electrophoresis on cellulose gel strips is suggested as a method for estimation of the number of carboxyl groups in the molecule and for separation of mono and polycarboxyl mercaptoacids. As a result of cyanamidation the molecule gains an additional positive charge reflected in a shift of pH‐mobility curves along the mobility axes.
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