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Transferrin and plasminogen phenotyping of aging blood stains using isoelectric focusing and silver stain
Author(s) -
Dykes Dale D.,
Miller Shirley A.,
Polesky Herbert F.
Publication year - 1985
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150060207
Subject(s) - isoelectric focusing , coomassie brilliant blue , staining , blood stains , stain , silver stain , chromatography , chemistry , transferrin , microbiology and biotechnology , agarose , biochemistry , biology , pathology , medicine , enzyme
A three‐month study was conducted on blood stains to determine the reliability and viability of the transferrin (Tf) and plasminogen (PLG) genetic markers upon storage under varying conditions. Stains stored at room temperature (19–22 °C), 4 °C and −70 °C were tested at weekly intervals. Isoelectric focusing on agarose gels was followed by fixation and staining with Coomassie Brilliant Blue R‐250. Gels were subsequently treated with silver stain if band intensity was too faint for phenotyping. Stains stored at room temperature and 4 °C showed a gradual decrease in band intensity. By using silver stain, it was possible to phneotype blood stains stored for at least twice the length of time as compared to those stained with Coomassie Blue. This study demonstrates that isoelectric focusing and silver staining is reliable for determining Tf and PLG markers in forensic investigations.