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Cell electrophoresis: Automatic measurements by laser light scattering with Lazypher
Author(s) -
Steiner Rudolf,
Ottmann Oliver,
Kaufmann Raimund,
Ann Light P.,
Hoffmann Walter
Publication year - 1985
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150060206
Subject(s) - myeloid leukemia , electrophoresis , spleen , myeloid , leukemia , microbiology and biotechnology , capillary electrophoresis , chemistry , cell , medicine , immunology , biology , biochemistry
Automated cell electrophoretic measurements with Lazypher are based on the laser Doppler technique. Experiments are performed with high precision (of 1.2% S. D.) and in short time intervals of 1–3 min. Application to electrophoretic mobility tests with tanned sheep erythrocytes incubated in supernatants from sensitized murine thymus and spleen cells which had been prepared from cultures containing 5 μg purified protein derivative (PPD)/ml caused a reduction of the electrophoretic mobility (EM) of 5% and 13%, respectively. Control spleen cell supernatants caused a 4% decrease in mobility and no effect was observed with supernatants from control thymus cells. Peripheral blood lymphocytes from healthy adults (n = 56) and children with atopic diseases and congenital heart disease (n = 12) are separated into a high mobility peak (μ = 1.24 μmcm/Vs for adults, μ = 1.19 μmcm/Vs for the child group) corresponding to T‐cells and a low mobility peak, μ = 0.93 μcm/Vs, representing B‐cells. The mobility difference between the adult and child T‐cells is highly significant (99%). Electrophoretic measurements of myeloid cells from healthy donors and patients with chronic myeloid leukemia (CML) and acute myeloid leukemia (AML) show that progressing differentiation within this cell line is accompanied by a decreasing mobility. Blast cells from a patient with acute lymphoblastic leukemia (c‐ALL) had an EM, μ = 1.09 μmcm/Vs, distinct from that of myeloblasts in AML (μ = 1.16 and 1.25 μmcm/Vs) and CML (μ = 1.25 and 1.23 μmcm/Vs) and from normal T‐and B‐lymphocytes.