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Non‐linear pH courses with immobilized pH gradients
Author(s) -
Gianazza Elisabetta,
Giacon Patrizia,
Sahlin Bengt,
Righetti Pier Giorgio
Publication year - 1985
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150060113
Subject(s) - isoelectric focusing , isoelectric point , immobilized ph gradient , range (aeronautics) , chemistry , ideal (ethics) , linear range , analytical chemistry (journal) , chromatography , resolution (logic) , distribution (mathematics) , biological system , computer science , materials science , mathematics , mathematical analysis , biochemistry , artificial intelligence , biology , detection limit , philosophy , epistemology , composite material , enzyme
Examples of increased resolution obtained with non‐linear pH gradients are presented. Discussed in detail is the “ideal” pH course in the range 4–10, based on the statistical distribution of isoelectric points of protein from data in the literature.