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Unconcentrated human urinary proteins analysed by high resolution two‐dimensional electrophoresis with narrow pH gradients: Preliminary findings after occupational exposure to cadmium
Author(s) -
Marshall Thomas,
Williams Katherine M.,
Vesterberg Olof
Publication year - 1985
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150060112
Subject(s) - isoelectric focusing , chemistry , chromatography , cadmium , silver stain , urine , resolution (logic) , urinary system , microbiology and biotechnology , biochemistry , medicine , endocrinology , biology , enzyme , artificial intelligence , computer science , organic chemistry
Over 600 polypeptides were detected in the two‐dimensional patterns of unconcentrated human urine following ultrasensitive silver staining. Resolution was greatly improved using different carrier ampholyte (Ampholine) mixtures of narrow pH range during isoelectric focusing. The effects of freezing and thawing, dialysis, concentration and different protein denaturing procedures were investigated. Preliminary studies of the urinary protein patterns of workers occupationally exposed to cadmium for many years showed a pronounced but selective increase in the number and amounts of polypeptides of M r <40 000.