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A new system for unloading gel rods for isoelectric focusing and a tank for running twenty second dimensional slab gels vertically
Author(s) -
Ramasamy Ragini,
Spragg Peter S.,
Jones Miren I.,
Amess Robert
Publication year - 1985
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150060111
Subject(s) - polyacrylamide , agarose , slab , rod , isoelectric focusing , buffer (optical fiber) , materials science , chromatography , chemistry , structural engineering , computer science , engineering , polymer chemistry , medicine , telecommunications , biochemistry , alternative medicine , pathology , enzyme
A stand is described which supports racks of gels for isoelectric focusing (ISO‐gels) and makes it possible to load these gel rods directly onto the second dimensional slabs of polyacrylamide (DALT‐gels). Equilibration with second dimensional buffer is made by adding it without agarose to the ISO‐gels on top of the slabs. A new design of DALT‐tank is also described to run twenty slabs of polyacrylamide while holding the plates vertically so the samples run down the slab. This system removes the need to seal the ISO‐gels onto the slab of polyacrylamide using agarose solutions. The omission of agarose reduces the background when two‐dimensional gels are stained with silver.