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Titration curves in ultrathin polyacrylamide and agarose gels
Author(s) -
Gunzer Gerhard
Publication year - 1984
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150050609
Subject(s) - agarose , polyacrylamide , titration , titration curve , chromatography , standard curve , chemistry , polyacrylamide gel electrophoresis , layer (electronics) , materials science , analytical chemistry (journal) , polymer chemistry , biochemistry , inorganic chemistry , organic chemistry , enzyme
A technique for the preparation of ultrathin titration curve gels, 50 μm polyacrylamide gels and 100 μm agarose gels, is presented. By separating protein standard mixtures the advantages of this ultrathin‐layer technique in terms of more convenient handling, cost and time are demonstrated.