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Dansylated proteins as internal standards in two‐dimensional electrophoresis and Western blot analysis
Author(s) -
Lubit Beverly W.
Publication year - 1984
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150050607
Subject(s) - polyacrylamide gel electrophoresis , isoelectric focusing , gel electrophoresis of proteins , isoelectric point , chromatography , chemistry , two dimensional gel electrophoresis , gel electrophoresis , electrophoresis , molecular weight size marker , blot , difference gel electrophoresis , biochemistry , proteomics , enzyme , gene
Dansylated proteins have been used in combination as isoelectric point and molecular weight standards and included in gel samples for separation by two‐dimensional polyacrylamide gel electrophoresis. These proteins provide invariant points of reference that facilitate the unequivocal identification of proteins separated by two dimensional polyacrylamide gel electrophoresis without affecting resolution. Moreover, immunoautoradiograms of Western blots can be aligned with their corresponding gel patterns with great accuracy. The logical extension of the established usage of dansylated protein standards to these applications is described.