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Some problems encountered in the selection of electrolyte systems in isotachophoresis
Author(s) -
Boček Petr,
Gebauer Petr
Publication year - 1984
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150050603
Subject(s) - electrolyte , isotachophoresis , stability (learning theory) , diagram , chemistry , selection (genetic algorithm) , chemical physics , biological system , thermodynamics , chromatography , computer science , mathematics , physics , statistics , biology , electrode , machine learning , artificial intelligence
A theory is described which gives a new algorithm for the selection of suitable electrolyte systems based on assuming (i) the zone stability as its correct isotachophoretic migration; (ii) the separability as the establishment of a fixed migration order of the zones of individual substances. The theory is based on data of the typeū i,j , i. e. , effective mobility of substance i in the j ‐th zone. For checking the stability of zones, the use of the zone existence diagrams is demonstrated, where the groups of substances giving stable zones are easily characterized, with special attention to anionic analyses with OH − as the terminator. For checking the separability of substances, the use of the sequence contours in an existence diagram is demonstrated with respect to the zone order as well as to the formation of stable mixed zones. Experiments are given where anionic isotachophoresis of some amino acids with OH − as the terminator is shown, and the formation of stable mixed zones of substances with mutually different effective mobilities is demonstrated.